- Sr Research Scientist - Bioinformatics (8/2005
to present) Promega Corporation
- Currently developing Windows forms
application for analysis of customer DNA quantitation data. This
application will import data in any of several formats, analyze
it according to customer settings and create control files for
further robotic processing of samples.
- Developed web applications for customer
use:
- Protease-Glo™ Oligonucleotide
Designer
Designs olgionucleotides for expression of protease cleavage
sites in GloSensor vectors (not yet available).
-
Cell ID™ Custom Bin Generator
Generates bin files
customized for specific custom capillary
electorphoresis machines (http://www.promega.com/techserv/tools/cellid/default.asp)
-
Blast Parser
Performs BLAST analysis of primers designed by
Plexor™ Primer Design System
and
analyzes results for potential mishybridization in real time
PCR assays (available after primer design at
https://www.promega.com/techserv/tools/plexor/logon.aspx
)
-
Assisted with
development, maintenance, troubleshooting, and testing of web
applications for customer use.
- Developed Database to manage:
- 1000 cDNA inserts Flexi vectors and
content as well as to perform in silico cloning experiments.
- Assisted R&D scientists, and others, in
general bioinformatics including:
- Primer design for PCR including
Promega products such as the Plexor® HY assay.
- Help with setting up spreadsheets and
macros for specific analyses
- Help with use of Vector NTI &
LaserGene
- ad hoc bioinformatics analyses as
needed
- Technical Services Scientist IV
(9/2003-8/2005) Promega Corporation.
- Provided technical support for Promega's
complete product line via telephone, Fax, and e-mail.
- Attended conferences as a Promega
representative.
- Provided technical training for new
Technical Services Scientists, Field Applications Specialists,
and various other Promega employees.
- Followed ISO9000 procedures.
- Lead internal teams to database Promega's
technical service information resources and rewrite the
technical appendix section of Promega's print catalog.
- Provided computer support for the
technical service department.
- Developed a number of web based tools for
biology (for example
http://www.promega.com/siRNADesigner/default.htm ).
- Extensive computer use including:
-
FrontPage Explorer & Editor
- HTML
-
JavaScript
-
VBscript
-
Microsoft Word
-
Bioinformatics servers on the web
-
Microsoft Internet Explorer
-
Microsoft Outlook, Microsoft
- Excel
- Access
- A
number of different internal software package
- Technical Services Scientist III
(9/2000-9/2003) Promega Corporation.
- Provided technical support for Promega's
complete product line via telephone, Fax, and e-mail.
- Attended conferences as a Promega
representative.
- Served on the High Throughput Screening
Business Unit Team.
- Wrote and Edited technical documents (for
instance
Screening for Genetically Modified Organisms in Food Using
Promega's Wizard? Resin)
- Provided technical training for new
Technical Services Scientists, Field Applications Specialists,
and various other Promega employees.
- Followed ISO9000 procedures.
- Designed web pages for Promega's internal
and external web sites using FrontPage.
- Provided computer support for the
technical service department.
- Learned JavaScript, VBscript, SQL, and
developed a number of web based tools for biology (for example
www.promega.com/biomath
www.promega.com/transfectionasst ,
www.promega.com/ctx,
http://www.promega.com/readit/probedesign/default.asp,
http://www.promega.com/apoasst/, and
www.promega.com/cellprolif ).
- Maintained the
FAQ,
Biomath,
Transfectionasst, and
Biolink, sections of Promega's external web page.
- Served on the Internet Team.
- Served on Gateway (Intranet) Team
- Served as a "Computer Wizard"
- Extensive computer use including:
-
FrontPage Explorer & Editor,
- HTML
-
JavaScript
-
Microsoft Word
-
Bioinformatics servers on the web
-
Microsoft Internet Explorer
-
Microsoft Outlook, Microsoft
- Excel
- Access
- A
number of different internal software package
- Technical Services Scientist II (9/98-9/2000)
Promega Corporation.
- Provided technical support for Promega's
complete product line via telephone, Fax, and e-mail.
- Wrote and edited literature for Promega
- Developed and maintained web pages for
Promega's internal and external web sites.
- Technical Services Scientist I (10/97-9/98)
Promega Corporation. Provided technical support for Promega's
complete product line via telephone, Fax, and e-mail. Designed web
pages for Promega's internal web site.
- Postdoctoral Research Associate (8/96-10/97).
Dr. Schuyler Korban. Department of Environmental Sciences and
Natural Resources, University of Illinois at Urbana/Champaign.
Investigated fatty acid synthesis in soybean: expression of genes
involved in fatty acid synthesis and modification during soybean
embryo development. Cloned fatty acid synthase genes. Developed a
lab web page (No longer available)
- Visiting Instructor (8/95-5/96).
Department of Biology,
St. Mary's College of Maryland. Teaching at a small liberal arts
college; directed independent student research projects,
participated in departmental and campus-wide faculty meetings,
advised students, and developed and taught courses.
·
Courses Taught
- Contemporary Bioscience (Fall 1995).
Introductory biology for non-majors.
- Contemporary Bioscience (Emphasis on
agricultural technology)(Spring 1996). This course focused on
various technologies, ranging from methods for sustainable
agriculture to the use of genetic engineering in crop plants,
used in agriculture.
- Biology of Plants (Spring 1996).
Introductory botany with laboratory. Physiology, anatomy,
development, genetics, and diversity of higher and lower plants
and fungi.
- Plant Physiology (Fall 1995). General
plant physiology. Original research papers relevant to plant
physiology were read and discussed in class. Laboratory
exercises were designed to give the students some familiarity
with modern plant physiology techniques (i.e. PCR and tissue
culture) and instrumentation (i.e. porometers and
pyschrometers).
- Introductory Biology Laboratory (Fall
1995, and Spring 1996). Laboratory for introductory biology for
biology majors
- Graduate Research Assistant (9/90- 8/95). Dr.
Stellos Tavantzis. Department of Plant Biology & Pathology,
University of Maine. Studied molecular genetics of Potato Virus X
(PVX) and PVX resistance in potato. Studied mycovirus genome
structure and expression using VSB717, a double-stranded RNA virus
of the plant pathogenic fungus Rhizoctonia solani, as a model
system. Computer analysis of DNA, RNA, and protein sequences.
Trained professors, graduate students, postdocs, and technicians in
a variety of computer techniques including the use of PCgene, BLAST
DNA homology search programs, and various internet tools. Trained
undergraduate lab assistants in molecular biology techniques.
- Teaching Assistant. Introductory Botany
(1/95-5/95). Dr. Christa Schwintzer and Dr. Benedict Neubauer.
Department of Plant Biology & Pathology, University of Maine. Taught
plant physiology and plant anatomy laboratories
- Laboratory Coordinator. Introductory Botany
(for students with no biology background) (8/94-12/94). Dr. Douglas
Gelinas. Department of Plant Biology & Pathology, University of
Maine. Designed, scheduled, and taught introductory botany
laboratories .
- Teaching Assistant. Introductory Biology
Laboratory (4 semesters between 1992 and 1994). Departments of Plant
Biology & Pathology, and Zoology, University of Maine. Presented
laboratory lectures, designed and graded all lab assignments.
Designed of molecular biology and immunology labs for all laboratory
sections (about 800 students).
- Research Assistant (1/88-8/90). Dr. Rodney
Sparks. Department of Cell Biology and Anatomy, Oregon Health
Science University. Studied molecular and cellular biology of
differentiation and oncogenesis of murine cells in culture. Trained
and supervised lab aides and summer interns in basic cell culture
and molecular biology techniques.
- Laboratory Assistant (summers '86 & '87). Dr.
John Hearst. Department Chemistry, University California at
Berkeley. Studied photosynthesis in photosynthetic bacterium
Rhodobacter capsulata. Attempted to develop monoclonal
antibodies for research on nucleic acid structure.
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Skills
- Programming for the web in C#.Net 2.0 & .Net
1.1
- Programming and using VBA macros in Microsoft
Excel & Microsoft word for DNA & protein analysis
- Programmatic manipulation and analysis of DNA,
RNA, and Protein sequences.
- Performing DNA analysis with a variety of
commercial and freely available tools and packages
- Use of Microsoft Excel for data analysis
- Use of NCBI (Genbank) data, web site, and web
services.
- SQL Server database use.
- Microsoft office use.
- Strong molecular biology skills including:
Plasmid DNA isolation, Genomic DNA isolation, RNA isolation,
Northern blotting, work with radioactivity, restriction digestion,
cDNA and genomic DNA library construction and screening, Restriction
digestion, in vitro transcription, DNA sequencing with the Sanger
method, Mammalian cell culture,
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