- Sr Research Scientist - Bioinformatics (8/2005 to
present) Promega Corporation
- Translated, liaised, and facilitated communication
between Information Technology and Scientific personnel.
- Scientific lead for:
- Scientific lead and sole programmer for:
- Developed numerous applications with Windows and Web
interfaces for internal use. These applications used primarily
Microsoft SQL Server and C#.Net technology and include:
- Analysis of stutter and peak balance in Short Tandem
Repeat data.
- Analysis of Small Pool PCR data.
- Analysis of various aspects of Mass Spec data.
- Analysis of Codon usage and codon pair frequency
- Detection and annotation of features on DNA and
protein sequences
- Access to web services from NCBI and EMBL
- Microsoft SQL Server database and interface to manage ~7000
cDNA inserts and vectors as well as to perform in silico
cloning experiments.
- Extensive work on search for human cDNA clones to
allow search by gene name, gene alias, accession number, sequence, and
keywords. This includes tools for comparison to standard (RefSeq)
sequences for the Gene of interest.
- Microsoft Word and Excel additions providing extensive
DNA and protein analysis capabilities and data analysis capabilities.
- Assisted R&D scientists, and others, in general
bioinformatics including:
- Primer design for PCR including Promega products such
as the Plexor® HY assay.
- Design of spreadsheets and macros for specific data analyses.
- Help with use of Vector NTI & LaserGene.
- ad hoc bioinformatics analyses as needed.
- Technical Services Scientist IV (9/2003-8/2005) Promega
Corporation.
- Led internal team to database Promega's technical
service information resources
- Led internal team rewrite the technical appendix
section of Promega's print catalog.
- Provided technical support for Promega's complete
product line via telephone, Fax, and e-mail.
- Attended conferences as a Promega representative.
- Provided technical training for new Technical Services
Scientists, Field Applications Specialists, and various other Promega
employees.
- Followed ISO9000 procedures.
- Provided computer support for the technical service
department.
- Developed a number of web based tools for biology
- Extensive computer use including:
- FrontPage Explorer
& Editor
- HTML
- JavaScript
- VBscript
- Microsoft Word
- Bioinformatics servers
on the web
- Microsoft Internet
Explorer
- Microsoft Outlook,
Microsoft
- Excel
- Access
- A number of different
internal software package
- Technical Services Scientist III (9/2000-9/2003) Promega
Corporation.
- Provided technical support for Promega's complete
product line via telephone, Fax, and e-mail.
- Attended conferences as a Promega representative.
- Served on the High Throughput Screening Business Unit
Team.
- Wrote and Edited technical documents (for instance Screening
for Genetically Modified Organisms in Food Using Promega's Wizard®
Resin)
- Provided technical training for new Technical Services
Scientists, Field Applications Specialists, and various other Promega
employees.
- Followed ISO9000 procedures.
- Designed web pages for Promega's internal and external
web sites using FrontPage.
- Provided computer support for the technical service
department.
- Learned JavaScript, VBscript, SQL, and developed
a number of web based tools for biology .
- Maintained the FAQ, Biomath, Transfectionasst, and Biolink,
sections of Promega's external web page.
- Served as a "Computer Wizard"
- Extensive computer use including:
- JavaScript
- Microsoft Office
- Bioinformatics servers
on the web
- A number of different
internal software package
- Technical Services Scientist II (9/98-9/2000) Promega
Corporation.
- Provided technical support for Promega's complete
product line via telephone, Fax, and e-mail.
- Wrote and edited literature for Promega
- Developed and maintained web pages for Promega's
internal and external web sites.
- Technical Services Scientist I (10/97-9/98) Promega
Corporation. Provided technical support for Promega's complete product
line via telephone, Fax, and e-mail. Designed web pages for Promega's
internal web site.
- Postdoctoral Research Associate (8/96-10/97). Dr.
Schuyler Korban. Department of Environmental Sciences and Natural
Resources, University of Illinois at Urbana/Champaign. Investigated
fatty acid synthesis in soybean: expression of genes involved in fatty acid
synthesis and modification during soybean embryo development. Cloned
fatty acid synthase genes. Developed a lab web page (No longer
available)
- Visiting Instructor (8/95-5/96). Department of Biology, St. Mary's College of Maryland. Teaching
at a small liberal arts college; directed independent student research
projects, participated in departmental and campus-wide faculty meetings,
advised students, and developed and taught courses.
· Courses
Taught
- Contemporary Bioscience (Fall 1995). Introductory
biology for non-majors.
- Contemporary Bioscience (Emphasis on agricultural
technology) (Spring 1996). This course focused on various technologies,
ranging from methods for sustainable agriculture to the use of genetic
engineering in crop plants, used in agriculture.
- Biology of Plants (Spring 1996). Introductory botany
with laboratory. Physiology, anatomy, development, genetics, and
diversity of higher and lower plants and fungi.
- Plant Physiology (Fall 1995). General plant physiology.
Original research papers relevant to plant physiology were read and
discussed in class. Laboratory exercises were designed to give the
students some familiarity with modern plant physiology techniques (i.e.
PCR and tissue culture) and instrumentation (i.e. porometers and
pyschrometers).
- Introductory Biology Laboratory (Fall 1995, and Spring
1996). Laboratory for introductory biology for biology majors
- Graduate Research Assistant (9/90- 8/95). Dr. Stellos
Tavantzis. Department of Plant Biology & Pathology, University of
Maine. Studied molecular genetics of Potato Virus X (PVX) and PVX
resistance in potato. Studied mycovirus genome structure and expression
using VSB717, a double-stranded RNA virus of the plant pathogenic fungus
Rhizoctonia solani, as a model system. Computer analysis of DNA,
RNA, and protein sequences. Trained professors, graduate students,
postdocs, and technicians in a variety of computer techniques including
the use of PCgene, BLAST DNA homology search programs, and various
internet tools. Trained undergraduate lab assistants in molecular
biology techniques.
- Teaching Assistant. Introductory Botany (1/95-5/95). Dr.
Christa Schwintzer and Dr. Benedict Neubauer. Department of Plant
Biology & Pathology, University of Maine. Taught plant physiology
and plant anatomy laboratories
- Laboratory Coordinator. Introductory Botany (for
students with no biology background) (8/94-12/94). Dr. Douglas Gelinas.
Department of Plant Biology & Pathology, University of Maine.
Designed, scheduled, and taught introductory botany laboratories .
- Teaching Assistant. Introductory Biology Laboratory (4
semesters between 1992 and 1994). Departments of Plant Biology &
Pathology, and Zoology, University of Maine. Presented laboratory
lectures, designed and graded all lab assignments. Designed of molecular
biology and immunology labs for all laboratory sections (about 800
students).
- Research Assistant (1/88-8/90). Dr. Rodney Sparks.
Department of Cell Biology and Anatomy, Oregon Health Science University.
Studied molecular and cellular biology of differentiation and
oncogenesis of murine cells in culture. Trained and supervised lab aides
and summer interns in basic cell culture and molecular biology
techniques.
- Laboratory Assistant (summers '86 & '87). Dr. John
Hearst. Department Chemistry, University California at Berkeley. Studied
photosynthesis in photosynthetic bacterium Rhodobacter capsulata.
Attempted to develop monoclonal antibodies for research on nucleic acid
structure.
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Skills
- Programming in C#.Net 3.5, 2.0 & .Net 1.1 including
extensive use of SOAP based web services to access data from NCBI and
similar data repositories.
- Programming and using VBA macros and vsto in Microsoft
Excel & Microsoft word for DNA & protein analysis
- Programmatic manipulation and analysis of DNA, RNA, and
Protein sequences.
- Performing DNA analysis with a variety of commercial and
freely available tools and packages
- Advanced use of Microsoft Excel for data analysis.
- Use of NCBI (Genebank) data, web site, and web services.
- SQL Server database use.
- Microsoft office use.
- Strong molecular biology skills including: Plasmid DNA
isolation, Genomic DNA isolation, RNA isolation, Northern blotting, work
with radioactivity, restriction digestion, cDNA and genomic DNA library
construction and screening, Restriction digestion, in vitro
transcription, DNA sequencing with the Sanger method, Mammalian cell
culture.
Patents
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